The Effect of Lignin on Enzyme Hydrolysis and Fermentation

• Barrier to cellulase enzymes
  – limit the efficacy of hydrolysis
• Adsorb cellulases
  –Increase enzyme loading and therefore the cost
• Phenloc compounds partition into biological membrances cause loss of integrity, therefore affecting their ability to serve selective barriers and enzyme matrices.

Less heavily substituted phenolics are the most inhibitory compounds
–Phenols
–Vanillin
–Hydroxylbenzaldehyde