In planta enzyme expression uses plants instead of microbial bioreactors as a “factory” to produce industrial hydrolytic enzymes for biofuel production. The enzymes that are active at typical and ambient plant growth temperatures will hurt plant normal growth/phenotypes and produce shriveled seeds as reported in the research paper.
However, in planta expression of thermophilic enzymes have been suggested as a method to reduce the detrimental effects on plants as these enzymes have higher temperature optima than encountered during plant growth. As a result, the plant stover can be pretreated at a relatively high temperature to induce their activation during processing.
Since a typical enzyme cocktail will require a combination of different class of enzymes to work together to destroy the cell wall for hydrolysis, is it possible to find all the key enzymes with thermophilibility and express them into a single plant without impacting plant growth?
However, in planta expression of thermophilic enzymes have been suggested as a method to reduce the detrimental effects on plants as these enzymes have higher temperature optima than encountered during plant growth. As a result, the plant stover can be pretreated at a relatively high temperature to induce their activation during processing.
Since a typical enzyme cocktail will require a combination of different class of enzymes to work together to destroy the cell wall for hydrolysis, is it possible to find all the key enzymes with thermophilibility and express them into a single plant without impacting plant growth?
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