Continuous cultivation process has been developed to answer the need of rapid ethanol production for fuels and chemical in the near future.
Batch cultivation is rarely applied for dilute-acid hydrolyzate cultivation since the cells is exposed directly to the high concentration level of inhibitors. This problem is commonly overcome by applying fed-batch cultivation where the concentrations of inhibitors in the media are controlled by regulation of the feeding. The continuous mode of cultivation has generally been of interest to minimize the total time of fermentation and increase the volumetric production rate of ethanol as well as decrease the investment cost. Because the bioreactor is fed continuously, it would be possible to avoid sudden inhibition to the yeast. Therefore, continuous cultivation mode is a suitable alternative for large-scale ethanol production.
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